The following local authorities have signed the Durban Commitment, and pledged their support for sustainable urban biodiversity management:

City of Amsterdam
City of Barcelona
Bergrivier Municipality
City of Bonn
Brussels Capital Region
Buffalo City Municipality
City of Bulawayo
City of Cape Town
Cape Winelands District Municipality
Commune de Fongo-Tongo
City of Curitiba
Dar es Salaam City Council
Douala IV Urban Council
Eden District Municipality
City of Edmonton
Ekurhuleni Metropolitan Municipality
Ethekwini Municipality
Ile de France
City of Johannesburg
City of Joondalup
Kasese District Local Government
King County
City of Leicester
City of Liverpool
Mexico City
Moshi Municipal Council
Motheo District Municipality
City of Nagoya
Nelson Mandela Bay Municipality
Municipality of Nioro du Rip
Municipality of Saint- Louis
City of São Paulo
Sedibeng District Municipality
City of Seoul
Stellenbosch Municipality
Steve Tshwete Local Government
City of Tilburg
City of Waitakere
Municipality of Walvis Bay
City of Zagreb